SK-MEL-5 人皮肤黑色素,瘤细胞

Catalogue No.: C352

Product Format: a T25 flask

Culture Properties：贴壁

Complete Growth Medium: 89%H-DMEM+10%FBS+1%双抗

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Application: Cells and cancer research

NOTE: FOR RESEARCH USE ONLY.

Components

Operation steps for cell culture

1. 吸走用于培养基，加入3-5mL PBS后轻轻晃动培养瓶润洗细胞；

3. 加入6-8ml完全培养基终止消化，轻轻吹下细胞混匀；

4. 将混匀的细胞1000rpm（约150g）离心3min，弃上清，再用新鲜培养基重悬37度培养箱继续培养；

传代比例: 不同细胞生长速度不一，具体传代比例视细胞生长速度而定，大部分细胞适用1:3-1:4传代，生长较慢的细胞可以1:2传代。

Macroautophagy and T-cell survival

Macroautophagy regulates T-cell survival at different levels. Dysregulated organelle

accumulation in the cytoplasm may act as an inducer of cell death in macroautophagy-

deficient T-cells, possibly as a consequence of increased generation of ROS caused by

impaired mitophagy [26, 50, 53]. However, the involvement of macroautophagy in the

regulation of apoptosis goes beyond mitochondrial homeostasis, as shown by the fact that Beclin-1 deficient T-cells present increased susceptibility to apoptosis, at least in part,