产地 | 进口/国产 |
保存条件 | 2-8℃,避光防潮 |
品牌 | 上海富雨 |
货号 | WM-YX10143 |
用途 | 仅供科研,不得用于临床 |
检测方法 | 竞争法/夹心法 |
保质期 | 6个月 |
适应物种 | 标题物种 |
检测限 | 可根据客户要求调整 |
数量 | 999 |
英文名称 | 人白介素3(IL-3)ELISA试剂盒 |
包装规格 | 48T/96T |
标记物 | HRP |
样本 | 血清、血浆、细胞上清液、组织匀浆 |
应用 | 仅供科研,不得用于临床 |
是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并 洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
engulfment of cytoplasmic cargo by lysosomes (or the vacuole in plants and fungi) and can either be non-selective, or selective, as in the cases of micropexophagy (degradation of peroxisomes), micromitophagy (degradation of mitochondria), and piecemeal microautophagy of the nucleus (PMN) (2 ). CMA involves the selective targeting of soluble cytosolic proteins to lysosomes for degradation. Proteins targeted by CMA have a common pentapeptide motif (KFERQ) which is recognized by the chaperone protein Hsc70 (heat shock cognate protein of 70 KDa) (3) .