产地 | 进口/国产 |
保存条件 | 2-8℃,避光防潮 |
品牌 | 上海富雨 |
货号 | WM-YX10142 |
用途 | 仅供科研,不得用于临床 |
检测方法 | 竞争法/夹心法 |
保质期 | 6个月 |
适应物种 | 标题物种 |
检测限 | 可根据客户要求调整 |
数量 | 999 |
英文名称 | 人白介素4(IL-4)ELISA试剂盒 |
包装规格 | 48T/96T |
标记物 | HRP |
样本 | 血清、血浆、细胞上清液、组织匀浆 |
应用 | 仅供科研,不得用于临床 |
是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
Macroautophagy involves the formation of double-membrane vesicles, termed autophagosomes, around a portion of cytoplasm, including organelles such as damaged/leaky mitochondria (e.g., mitophagy), misfolded or aggregated proteins (e.g., aggrephagy) or microbes (e.g., xenophagy) (4 ). Autophagosomes are then able to fuse with functional lysosomes (“maturation” or “lux”) for degradation of the luminal contents.
Macroautophagy (herein, called autophagy) is a fundamental, homeostatic process integrated into both the innate and adaptive arms of the immune system (4, 5 ). The immunological functions of autophagy appear to interact and modify “classical” innate immune pathways including (i) direct microbial elimination; (ii) cooperation with pattern recognition receptors (PRR); (iii) inlammasome regulation; (iv) secretion of biomolecules; and (v) major histocompatibility complexes (MHC) and antigen processing (4 –6 ). In addition, autophagy plays a major role in B and T cell activation, proliferation and survival as well as afects positive and negative thymic selection of na?ve T cells (7, 8) .