产地 | 进口/国产 |
保存条件 | 2-8℃,避光防潮 |
品牌 | 上海富雨 |
货号 | WM-YX10140 |
用途 | 仅供科研,不得用于临床 |
检测方法 | 竞争法/夹心法 |
保质期 | 6个月 |
适应物种 | 标题物种 |
检测限 | 可根据客户要求调整 |
数量 | 999 |
英文名称 | 人白介素6(IL-6)ELISA试剂盒 |
包装规格 | 48T/96T |
标记物 | HRP |
样本 | 血清、血浆、细胞上清液、组织匀浆 |
应用 | 仅供科研,不得用于临床 |
是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
CROSSTALK BETWEEN AUTOPHAGY AND
INNATE PATHWAYS TO INFLUENCE T CELL RESPONSES
The above immunological functions of autophagy represent the interplay and interaction of this cellular process within a single cell (e.g., pathogen elimination in APC; proliferation through clearance of cell cycle proteins in T cells). This section seeks to discuss the physiological and immunological relevance of autophagy and how it interacts at a systemic level—whereby autophagy in innate immune cells (e.g., DC and macrophages) afects CD8+ and CD4+ T helper (TH) cell responses. This type of crosstalk may play a critical role in the pathogenesis of various diseases such as Crohn’s disease (CD), rheumatoid arthritis (RA) and multiple sclerosis (MS) as well as chronic infections like tuberculosis (TB).