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| 产地 | 进口/国产 |
| 保存条件 | 2-8℃,避光防潮 |
| 品牌 | 上海富雨 |
| 货号 | WM-YX10123 |
| 用途 | 仅供科研,不得用于临床 |
| 检测方法 | 竞争法/夹心法 |
| 保质期 | 6个月 |
| 适应物种 | 标题物种 |
| 检测限 | 可根据客户要求调整 |
| 数量 | 999 |
| 英文名称 | 人血血小板衍生生长因子可溶性受体α(PDGFsR-α)ELISA试剂盒 |
| 包装规格 | 48T/96T |
| 标记物 | HRP |
| 样本 | 血清、血浆、细胞上清液、组织匀浆 |
| 应用 | 仅供科研,不得用于临床 |
| 是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
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Thus, loss of autophagy, and more speciically mitophagy (a form of autophagy that sequesters and degrades mitochondria) (50 ), in macrophages and DC, combined with inlammatory stimulation, can lead to the accumulation of damaged/dysfunctional mitochondria. This chain of events leads to the release of mitochondrial ROS and DNA into the cytosol, which in turns activates the NLRP3 inlammasome, leading to increased caspase-1-dependent processing and release of IL-1β and IL-18. However, the link between autophagy and inlammasome regulation does not end there (Figure 2) .
