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| 产地 | 进口/国产 |
| 保存条件 | 2-8℃,避光防潮 |
| 品牌 | 上海富雨 |
| 货号 | WM-YX10118 |
| 用途 | 仅供科研,不得用于临床 |
| 检测方法 | 竞争法/夹心法 |
| 保质期 | 6个月 |
| 适应物种 | 标题物种 |
| 检测限 | 可根据客户要求调整 |
| 数量 | 999 |
| 英文名称 | 人可溶性CD40配体(sCD40L)ELISA试剂盒 |
| 包装规格 | 48T/96T |
| 标记物 | HRP |
| 样本 | 血清、血浆、细胞上清液、组织匀浆 |
| 应用 | 仅供科研,不得用于临床 |
| 是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被弹性蛋白（elastin）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并 洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的弹性蛋白（elastin）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。
suggested for inlammasomes. Induction of autophagy during or after priming of cells with a TLR agonist results in a reduction of intracellular pro-IL-1β (38 ), but it is unclear whether it is
degraded in the autophagosomes, or whether it is transported elsewhere for proteasomal degradation, which appears to be the more common route of degradation for this cytokine (55) . However, autophagy may also have a role to play in the secretion of IL-1β. While most secreted proteins have an N-terminal signal peptide that allows them to tra伍c through the classical
