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| 产地 | 进口/国产 |
| 保存条件 | 2-8℃,避光防潮 |
| 品牌 | 上海富雨 |
| 货号 | WM-YX10090 |
| 用途 | 仅供科研,不得用于临床 |
| 检测方法 | 竞争法/夹心法 |
| 保质期 | 6个月 |
| 适应物种 | 标题物种 |
| 检测限 | 可根据客户要求调整 |
| 数量 | 999 |
| 英文名称 | 人细胞间粘附分子3(ICAM-3/CD50)ELISA试剂盒 |
| 包装规格 | 48T/96T |
| 标记物 | HRP |
| 样本 | 血清、血浆、细胞上清液、组织匀浆 |
| 应用 | 仅供科研,不得用于临床 |
| 是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
Furthermore, in vitro treatment of na?ve murine CD3+ T cells with supernatants from LPS and 3-MA-treated dendritic cells (high in IL-1α, IL-1β, and IL-23), enhances secretion of IL-17A, IL-17F, IFN-γ, and IL-22 (54) .
Regarding TH17 cells, these CD4+ T cells play an important role in controlling extracellular bacteria and fungi by producing the cytokines IL-17A and IL-17F that act on epithelial cells to recruit neutrophils. TH17 cells and cytokines associated with TH17 responses have also been implicated in numerous autoimmune and infectious diseases (69, 70 ). In fact, several studies conirm that autophagy disruption in APC during infection leads to excessive inlammation which is associated with
