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| 产地 | 进口/国产 |
| 保存条件 | 2-8℃,避光防潮 |
| 品牌 | 上海富雨 |
| 货号 | WM-YX10078 |
| 用途 | 仅供科研,不得用于临床 |
| 检测方法 | 竞争法/夹心法 |
| 保质期 | 6个月 |
| 适应物种 | 标题物种 |
| 检测限 | 可根据客户要求调整 |
| 数量 | 999 |
| 英文名称 | 人巨噬细胞移动抑制因子(MIF)ELISA试剂盒 |
| 包装规格 | 48T/96T |
| 标记物 | HRP |
| 样本 | 血清、血浆、细胞上清液、组织匀浆 |
| 应用 | 仅供科研,不得用于临床 |
| 是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被颗粒酶B(Gzms-B)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的颗粒酶B(Gzms-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
Autophagy Regulates T Cell Survival and
Proliferation
Over the last decade, several groups have conirmed autophagy as an active cellular process in T cells (106 –112 ). Initially, the conirmation of this pathway, detection of autophagosomes and up-regulation of autophagy proteins, was only observable in T cells after T cell receptor (TCR) stimulation. It was further substantiated to be an active process in na?ve T cells due to the expression of numerous autophagy genes (Atg5, Atg7, Atg8, and Beclin-1) found prior to stimulation. To further understand the role of autophagy in T cells, several groups generated numerous T cell-speciic conditional knockout mice to delete various
