产地 | 进口/国产 |
保存条件 | 2-8℃,避光防潮 |
品牌 | 上海富雨 |
货号 | WM-YX10068 |
用途 | 仅供科研,不得用于临床 |
检测方法 | 竞争法/夹心法 |
保质期 | 6个月 |
适应物种 | 标题物种 |
检测限 | 可根据客户要求调整 |
数量 | 999 |
英文名称 | 人血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 |
包装规格 | 48T/96T |
标记物 | HRP |
样本 | 血清、血浆、细胞上清液、组织匀浆 |
应用 | 仅供科研,不得用于临床 |
是否进口 | 否 |
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被乙醇脱氢酶(ADH)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并 洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的乙醇脱氢酶(ADH)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品活性。
Given that TCR and cytokine stimulation can activate autophagy it is easy to speculate autophagy has a hand in CD4+ T cell diferentiation and function. However, autophagy’s inluence on T cell diferentiation and function has remained unclear for many years due to autophagy’s critical role in T cell survival (107, 112, 124, 125 ). Moreover, it appears there is a varying degree to which each subset of CD4+ T cells utilize autophagy for diferentiation and function. For example, autophagy appears
Cuervo, A. M., and Macian, F. (2014). Autophagy and the immune function in aging. Curr. Opin. Immunol. 29, 97–104.
Deretic, V., and Levine, B. (2018). Autophagy balances in?ammation in innate immunity. Autophagy 14, 243–251.