检测原理

检测原理

试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被丝氨酸蛋白酶8（PRSS8）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并 洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的丝氨酸蛋白酶8（PRSS8）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。

and  Vander  Heiden   (149 )  report  that  the  shift  toward  glycolytic anabolism during the active state supports the needs  of proliferation  across  cell  types  by  generating  the  materials  required  to  produce  new  daughter  cells  (149) .  Activated  T  cells  have  been  shown  to  rely  on  OXPHOS  to  support  early  proliferation  and  cytokine production.  Glycolytic-incompetent  T

the information of drugs targeting these enzymes in tumor immunotherapy.The microbiome is a potent modulator of host immune responses and over the past years has been shown to impact tumor immunology. Both pro-tumorigenic and anti-tumorigenic functions have been associated with the microbiome and functional studies have pinpointed specific anti-tumor immunity-promoting microbes, such as Akkermansia muciniphila and Bifidobacterium longum. The identification of key host genes and microbe-derived signals involved in anti-tumor immunity is still in its infancy. Here we focus on recent advances in this area, revealing host