检测原理

试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被丝氨酸蛋白酶8（PRSS8）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并 洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的丝氨酸蛋白酶8（PRSS8）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。

discovering novel agents as well as repurposing FDA-approved pharmaceuticals to modulate autophagy (194 –196 ). Below we discuss potential T cell-linked diseases that may beneit from  autophagy modulation.

molecules found to be central in host-microbiome dependent modulation of tumor immunity, and highlight key questions to be tackled in the field.Decision making in immuno-oncology is pivotal to adapt therapy to the tumor microenvironment (TME) of the patient among the numerous options of monoclonal antibodies or small molecules. Predicting the best combinatorial regimen remains an unmet medical need. Here, we report a multiplex functional and dynamic immuno-assay based on the capacity of the TME to respond to ex vivo stimulation with twelve immunomodulators including immune checkpoint inhibitors (ICI) in 43 human primary tumors.