种属 人
别称 CNE1; CNE
疾-病 鼻咽-癌
传代比例/细胞消化 1:2传代 ,消化2-3分钟
完全培养基配置 RPMI1640培养基;10%胎牛血清;1%双抗
简介 此细胞株来源于一位58岁女性鼻咽-癌患者。Problematic cell line: Contaminated. On the basis of the STR
profile (PubMed =18196576) it is identical to CNE-2 and seems to be a hybrid of Hela and of cell line of unknow origin. This is also confirmed by RNASeq data from PubMed =24991015. Originally thought to originate from a 58 year old female patient with a nasopharyngeal carcinoma.
形态 上皮细胞样
生长特征 贴壁生长
倍增时间 每周 2 至 3 次
STR Amelogenin:X;CSF1PO:9 ,10;D13S317:13.3;D16S539:9 ,10;D18S51:16;D19S433:13 ,14;
D21S11:27 ,28;D2S1338:17;D3S1358:15 ,18;D5S818:11 ,12;D7S820:8 ,12;D8S1179:12 ,13;
FGA:18 ,21;TH01:7;TPOX:8 ,12;vWA:16 ,18
培养条件 气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。
冻存条件 冻存液:90%FBS ,DMSO 10%,