种属 小鼠
别称 HL1
组织来源 小鼠心肌细胞
疾-病 转化细胞系
传代比例/细胞消化 1:2传代 ,消化2-3分钟
完全培养基配置 MEM培养基;10%胎牛血清;1%双抗
简介 HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including
morphological, biochemical, and electrophysiological properties. HL-1 is used in a variety of model systems to address questions of cardiac biology at the cellular & molecular levels.
形态 成纤维细胞样
生长特征 贴壁生长
倍增时间 每周 2 至 3 次
培养条件 气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。
冻存条件 冻存液 :90%FBS ,DMSO 10%,
或使用非程序冻存液 :官网货号JY-H040
保藏机构 Millipore;SCC065